Embryo Transfer

Bulletin:  Embryo Transfer (ET)and Donor Cows

Executive Summary:  if your donor cow was registered before January 1, 2011, you may have to provide a hair sample to complete a DNA profile on file so that her ET propagated calves may be eligible for registration.  This is not a new requirement but one often overlooked.

If you are new to using embryo transfer (ET) technology to propagate Speckle Park cattle here are some details you won’t want to miss.
a.   The donor cow is required to have a DNA profile on record with the Association’s registrar before a calf propagated by embryo transfer can be registered.
b.    The semen donor is required to have a DNA profile on record as well.
c.     The calf propagated by ET must qualify by means of a DNA profile as progeny of both sire and dam before it can be registered.   The calf’s DNA profile will be completed as part of the registration process.
d.   Timing.  The hair “samples from the donor cow must be collected no later than the time of transplanting” according to the By-law quoted below.

Further Notes: 
·   Since approximately 2000 all Speckle Park bulls have had to have a DNA profile on file with the Associations registrar in order to be registered.
·   All cows registered since January 1, 2011 will also have a DNA profile on file as a requirement for registration.
·   However, if the donor cow was registered before 2011, please ensure that a DNA profile is on file.  You can check the Speckle Park Pedigree Data through CLRC.  If there is no DNA profile on record, please complete one by requested a DNA Kit for the cow from our Registrar at CLRC.
·   As has been the practice with the bulls since approximately 2002, when the lab processes the hair and creates the new DNA profile, the lab automatically checks this new profile against the profiles of the sire and dam of record.  The sires will have DNA profiles on file, but the dams probably not.  There is no need for the donor cow to be DNA verified against her dam, if the donor’s dam does not have a DNA profile. 

Here are the two most relevant CSPA By-laws that have a direct impact on your use of embryo technology to propagate Speckle Park cattle.  This is verbatim quotation with underlining added for emphasis.

SECTION 20. ARTIFICIAL INSEMINATION AND EMBRYO TRANSPLANT

The following CSPA By-laws apply to all purebred, percentage and appendix Speckle Park registered by the Association.

a)  Calves conceived by artificial insemination will be registered on the same terms as calves conceived by natural mating, except a DNA genotype certificate for the sire must be on file with the Association’s Registrar.

b)  Calves propagated by embryo transplant will be registered on the same terms as calves propagated by natural or artificial mating, except a DNA Genotype Certificate for the sire, dam and calf must be on file with the Association’s Registrar, as must be a completed Application for Registration as supplied by the Association and an Embryo Transplant Certificate.

i) To verify parentage of embryo transplant offspring, the DNA Genotype Certificates of all sires used as semen sources must be on record with a recognized laboratory prior to the time of transplanting. The required samples from the donor cow must be collected no later than the time of transplanting, if not already on record.

ii)  It is hereby stressed that both Canadian Speckle Park Association’s members and Embryo Transplant centers be advised of the importance of being able to differentiate in instances when two bulls are being used to simultaneously inseminate donor cows. In those cases where parentage cannot be established through DNA Genotyping, the resultant offspring will not be eligible for registration in the Herd Book of the Canadian Speckle Park Association.

If you are considering using imported semen and/or embryos, please read the CSPA By-laws, SECTON 18. RECOGNITION OF SPECKLE PARK ASSOCIATIONS AND IMPORTED GENETICS

If you are wondering who is the breeder and who is the owner at birth of ET propagated calves, please read SECTION 21.  APPLICATION FOR REGISTRATION, sub-section 9).

To view these sections and the complete By-laws of the Canadian Speckle Park Association click here.

CSPA, August 16, 2016
Posted on CSPA Website October 6, 2016